中药成分检测报告(丹芎方)

项目报告
1 样品信息Sample description
收到丹芎方样品xx个,a和b
A total of xx danxiong prescription samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,丹参素,阿魏酸,隐丹参酮,丹参酮ⅡA标准品购于xxx.
All chemicals and solvent were HPLC. Standard: danshensu, ferulic acid, cryptotanshinone, tanshinone ⅡA and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取5.0g左右的样品;
2) 5.0g样品加入xxx助溶,生理盐水溶解并定容,密封,置于4℃冰箱中;
3) 量取配置好的xxx悬液100 µL,加甲醇至5 mL使其全部溶解,旋涡震荡30s,3000 r/min离心10 min;
4) 上清液并用0.45µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Agilent高效液相色谱仪,配备二极管阵列检测器。
色谱柱为Agilent xxx-C18 (150 mm*4.6 mm, 5 μm),流动相为xxx和0.5% xxx溶液,流速为1.0 mL/min。
3 结果Results
色谱图Chromatogram


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