中药成分检测报告(三七)
项目报告
1 样品信息Sample description
收到三七样品xx个,a和b
A total of xx Panax notoginseng samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,人参皂苷Rg1,人参皂苷Re,人参皂苷Rb1,三七皂苷R1标准品购于xxx
All chemicals and solvent were HPLC. Standard: ginsenoside Rg1, ginsenoside Re, geniposide Rb1, notoginsenoside R1 and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取8 mg左右的样品;
2) 8 mg样品中加入0.4 mL内标液(xxx 7.5mg用乙腈-xxx(81 : 19, V/V)溶解并定容至25 mL);
3) 加入乙腈-xxx(81 : 19, V/V)溶解并定容至25 mL,摇匀,并用0.45 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为LC高效液相色谱仪,配备紫外检测器。
色谱柱为xxx (250 mm*4.6 mm, 5 μm),流动相为乙腈-xxx(81 : 19, V/V),流速为1.2 mL/min,柱温为室温。
3 结果Results
色谱图Chromatogram
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