中药成分检测报告(百花蛇舌草)
项目报告
1 样品信息Sample description
收到百花蛇舌草样品xx个,a和b
A total of xx Herba Hedyotis diffusae samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,齐墩果酸,熊果酸标准品购于xxx
All chemicals and solvent were HPLC. Standard: oleanolic acid, ursolic acid and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.5g左右的样品;
2) 0.5g样品中加入20 mL xxx,超声提取30 min;
3) 抽滤,滤液用xxx定容至25 mL容量瓶中,摇匀并用0.45 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪,配备紫外检测器。
色谱柱为xxx C18 (250 mm*4.6 mm, 5 μm),流动相为甲醇-2% xxx(88 : 12,V/V),流速为1.0 mL/min,柱温为30℃。
3 结果Results
色谱图Chromatogram
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