植物黄酮检测报告(黄芩)
项目报告
1 样品信息Sample description
收到黄芩样品xx个,a和b
A total of xx scutellaria baicalensis samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,黄芩苷,汉黄芩苷,黄芩素,汉黄芩素,千层纸素A标准品购于xxx
All chemicals and solvent were HPLC. Standard: baicalin, wogonoside, baicalein, wogonin, oroxylin A and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.05g左右的样品;
2) 0.05样品加入50 mL 70% xxx,称定重量,超声提取(功率100W,频率40 kHz)1 h;
3) 放冷,称重,用70% xxx补足减失的重量,摇匀,过滤;
4) 滤液用0.22 µm滤膜过滤,进样10 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Waters高效液相色谱仪,配备二极管阵列检测器。
色谱柱为xxx C18 (250 mm*4.6 mm, 5 μm),流动相A为乙腈-水-xxx (21 : 78 : 1, V/V/V),流动相B为乙腈-水-xxx(80 : 20 : 1, V/V/V),流速为1.0 mL/min,柱温为30℃。
3 结果Results
色谱图Chromatogram
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