神经递质检测报告(小鼠)
项目报告
1 样品信息Sample description
收到小鼠样品xx个,a和b
A total of xx mouse samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,谷氨酸,天冬氨酸,γ-氨基丁酸,甘氨酸,牛磺酸标准品购于xxx
All chemicals and solvent were HPLC. Standard: glutamate, aspartate, γ- aminobutyric acid, glycine, taurine and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取一定量的样品;
2) 加入0.4 mol/L高氯酸,匀浆8 min;
3) 加入40 µL xxx,将匀浆液转至离心管中,用0.4 mol/L高氯酸洗涤匀浆器,洗液合并至离心管中,4℃下10000 r/min离心10 min;
4) 取上清液250 µL加入100 µL 2 mol/L xxx和200 µL xxx溶液,混匀,80℃水浴30 min;
5) 取出后,加入1 mol/L xxx溶液终止反应,冷却,经0.45µm滤膜过滤,进样20 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为xxx高效液相色谱仪,配备荧光检测器。
色谱柱为Kromasil C18 (250 mm*4.6mm, 5 μm),流动相为甲醇-0.1 mol/L xxx (42:58, V/V)混合溶液,流速为1.0 mL/min,柱温为室温。
3 结果Results
色谱图Chromatogram