核苷检测报告(北虫草)
![检测报告表 检测报告表](http://file.biotech-pack.com/upload/bt-dxt/image/article/nucleoside-sample-report-2.png?x-oss-process=style/dxt)
项目报告
1 样品信息Sample description
收到北虫草样品xx个,a和b
A total of xx cordyceps militaris samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,尿苷,肌苷,鸟苷,腺苷,虫草素,N6-(2-羟乙基)-腺苷标准品购于xxx
All chemicals and solvent were HPLC. Standard:uridine, inosine, guanosine, adenosine, cordycepin, N6-(2-hydroxyethyl)-adenosine and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.5g左右的样品;
2) 0.5g样品于60 mL平行提取管中,加入25 mL xxx,称量;
3) 涡旋混匀后,置于平行反应器中100℃,150 r/min提取30 min;
4) 补足失重,提取液13000 r/min离心5 min;
5) 上清液用0.45 µm滤膜过滤,进样5 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Agilent 高效液相色谱仪,配备二极管阵列检测器。
色谱柱为xxx C18 (150 mm*4.6 mm, 3 μm),流动相为A为水,B为xxx,流速为xxx mL/min,柱温为25℃。
3 结果Results
色谱图Chromatogram
![样品和标准品色谱图 样品和标准品色谱图](http://file.biotech-pack.com/upload/bt-dxt/image/article/nucleoside-sample-report-21.png?x-oss-process=style/dxt)
![北虫草中核苷测定结果 北虫草中核苷测定结果](http://file.biotech-pack.com/upload/bt-dxt/image/article/nucleoside-sample-report-22.png?x-oss-process=style/dxt)
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