核苷检测报告(药材2)
检测报告表

项目报告

1 样品信息Sample description
收到药材样品xx个,a和b
A total of xx medicinal materials samples, where received by the customer.

2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,腺苷,2, 4-二羟基嘧啶标准品购于xxx
All chemicals and solvent were HPLC. Standard: adenosine, 2, 4-dioxypyrimidine and were purchased from xxx.

2.2 样品处理Sample Preparation
1) 将样品摇匀,称取5.0g左右的样品;
2) 5.0g样品加入30 mL 75% xxx提取2次,每次1 h;
3) 除去乙醇,加入20 mL水溶解,并用10 mL xxx萃取,蒸发至干;
4) 加甲醇溶解并定容至50 mL,用0.45 µm滤膜过滤,进样20 µL。

2.3 HPLC分析HPLC Analysis
本实验所用的仪器为Agilent高效液相色谱仪,配备二极管阵列检测器。
色谱柱为xxx C18 (200 mm*4.6 mm, 5 μm),流动相为甲醇-xxx,流速为1.0 mL/min,柱温为25℃。

3 结果Results
色谱图Chromatogram

样品和标准品色谱图
药材中核苷测定结果

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