神经递质检测报告(生物组织)
项目报告
1 样品信息Sample description
收到生物组织样品xx个,a和b
A total of xx biological tissue samples, where received by the customer.
2 材料与方法Materials and Methods
2.1 试剂Chemicals
所有的溶剂与试剂均为色谱级,左旋多巴,去甲肾上腺素,肾上腺素,多巴柯,多巴胺,5-羟基吲哚乙酸,高香草酸,5-羟色胺标准品购于xxx
All chemicals and solvent were HPLC. Standard: L-dihydroxyphenylalanine, norepinephrine, eoubeogrube, 3, 4-hydroxyphenylacitic acid, dopamine, 5-hydroxyindole acetic acid, 4-hydroxy-3-methoxyacetic acid, 5-hydroxytryptamine and were purchased from xxx.
2.2 样品处理Sample Preparation
1) 将样品摇匀,称取0.5 g左右的样品;
2) 0.5 g样品加入组织裂解液(0.01% L-半胱氨酸,0.2mmol/L xxx,0.5 mmol/L xxx),超声破碎60分钟;
3) 4℃下离心10分钟,14000 r/min;
4) 上清过0.2 µm的xxx过滤器,进样20 µL。
2.3 HPLC分析HPLC Analysis
本实验所用的仪器为xxx型高效液相色谱仪, 配备xxx电化学检测器。
色谱柱为ESA MD-150 (150 mm*3.2 mm, 2 μm),流动相为5%~10% xxx,50 mmol/L柠檬酸、50 mmol/L xxx、0.5 mmol/L 1-xxx、0.5 mmol/L Na2EDTA、5 mmol/L三乙胺,流速为0.4 mL/min,柱温为30℃。
3 结果Results
色谱图Chromatogram